miRNA: 3'- uAUACaCAGUUGCGGCUCAcGUccAG- -5'
miRNA: 3'- uauaCAC-AGUUGCGGC-----UCACGuCCAG- -5'
miRNA: 3'- uauaCACAGuuGCGGCUC--ACGUCCAg -5'
miRNA: 3'- -UAUACACAGUUGCGGCUCACGUCCAG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 770 | 5' | -52.7 | NC_000852.3 | + | 328483 | 0.66 | 0.996963 |
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Target: 5'- uUGUGcGUCAACGCCGAGU-CA--UCa -3' miRNA: 3'- uAUACaCAGUUGCGGCUCAcGUccAG- -5' |
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| 770 | 5' | -52.7 | NC_000852.3 | + | 87250 | 0.68 | 0.982668 |
|
Target: 5'- ---gGUGaUCAACGCUGgggacGGUGCcGGUCa -3' miRNA: 3'- uauaCAC-AGUUGCGGC-----UCACGuCCAG- -5' |
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| 770 | 5' | -52.7 | NC_000852.3 | + | 286277 | 0.69 | 0.970827 |
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Target: 5'- ---gGUGUUggUGCCGGGgcUGUAGGUg -3' miRNA: 3'- uauaCACAGuuGCGGCUC--ACGUCCAg -5' |
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| 770 | 5' | -52.7 | NC_000852.3 | + | 328144 | 1.09 | 0.011556 |
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Target: 5'- aAUAUGUGUCAACGCCGAGUGCAGGUCa -3' miRNA: 3'- -UAUACACAGUUGCGGCUCACGUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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