Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7700 | 3' | -53.2 | NC_001973.1 | + | 22064 | 0.66 | 0.973115 |
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Target: 5'- uGGACUgGGCgG-CGUGUGCG-GAg- -3' miRNA: 3'- uUUUGAgCCGgCaGCACACGCaCUag -5' |
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| 7700 | 3' | -53.2 | NC_001973.1 | + | 97860 | 0.66 | 0.970243 |
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Target: 5'- gGAGACgaacagggGGCCGUCGUGgGCGUagcaGUCg -3' miRNA: 3'- -UUUUGag------CCGGCAGCACaCGCAc---UAG- -5' |
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| 7700 | 3' | -53.2 | NC_001973.1 | + | 23013 | 0.67 | 0.943844 |
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Target: 5'- aAAGAuCUUGGCCGaCGUG-GUGUGggCg -3' miRNA: 3'- -UUUU-GAGCCGGCaGCACaCGCACuaG- -5' |
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| 7700 | 3' | -53.2 | NC_001973.1 | + | 111284 | 0.68 | 0.92344 |
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Target: 5'- -cAACUCGGUgGUgGUGgcgccgcGCGUGAUg -3' miRNA: 3'- uuUUGAGCCGgCAgCACa------CGCACUAg -5' |
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| 7700 | 3' | -53.2 | NC_001973.1 | + | 14339 | 0.71 | 0.794794 |
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Target: 5'- -cAAC-CGGCCGcacuuggCGUGgucgGCGUGGUCg -3' miRNA: 3'- uuUUGaGCCGGCa------GCACa---CGCACUAG- -5' |
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| 7700 | 3' | -53.2 | NC_001973.1 | + | 112811 | 1.08 | 0.00579 |
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Target: 5'- cAAAACUCGGCCGUCGUGUGCGUGAUCa -3' miRNA: 3'- -UUUUGAGCCGGCAGCACACGCACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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