Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 771 | 5' | -48 | NC_000852.3 | + | 178925 | 0.66 | 0.999992 |
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Target: 5'- aGGAGAugcCCUCAa----GGCGUGGUGCUc -3' miRNA: 3'- -UCUCU---GGAGUaacugUCGUAUCAUGA- -5' |
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| 771 | 5' | -48 | NC_000852.3 | + | 112159 | 0.7 | 0.998739 |
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Target: 5'- uGAGuGCCgaugUAcgGACGGCGUAGUGCg -3' miRNA: 3'- uCUC-UGGa---GUaaCUGUCGUAUCAUGa -5' |
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| 771 | 5' | -48 | NC_000852.3 | + | 36599 | 0.7 | 0.998739 |
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Target: 5'- -cGGACCUCAUacgcuaGAaAGCAUGGUACa -3' miRNA: 3'- ucUCUGGAGUAa-----CUgUCGUAUCAUGa -5' |
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| 771 | 5' | -48 | NC_000852.3 | + | 81656 | 0.72 | 0.992469 |
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Target: 5'- --uGGCCUUGUUGGCAGCgAUAGUAg- -3' miRNA: 3'- ucuCUGGAGUAACUGUCG-UAUCAUga -5' |
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| 771 | 5' | -48 | NC_000852.3 | + | 324842 | 0.74 | 0.97971 |
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Target: 5'- gAGAGACCUUGUcGACAGCAUAu---- -3' miRNA: 3'- -UCUCUGGAGUAaCUGUCGUAUcauga -5' |
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| 771 | 5' | -48 | NC_000852.3 | + | 3051 | 1.08 | 0.034783 |
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Target: 5'- gAGAGACCUCAUUGACAGCAUAGUACUc -3' miRNA: 3'- -UCUCUGGAGUAACUGUCGUAUCAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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