Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7722 | 3' | -56.4 | NC_001973.1 | + | 4170 | 0.69 | 0.741018 |
Target: 5'- gGGUCCGUcGC--CGCGCauguuGAAACgCCGCAg -3' miRNA: 3'- -UCAGGCA-CGaaGCGCG-----CUUUG-GGCGU- -5' |
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7722 | 3' | -56.4 | NC_001973.1 | + | 31751 | 0.7 | 0.721498 |
Target: 5'- gGG-CCGUGCcgCGCGCGcuGCCCa-- -3' miRNA: 3'- -UCaGGCACGaaGCGCGCuuUGGGcgu -5' |
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7722 | 3' | -56.4 | NC_001973.1 | + | 108433 | 0.7 | 0.68162 |
Target: 5'- gAGagCGaGCggcgcUCGCGCGAcGCCCGCAc -3' miRNA: 3'- -UCagGCaCGa----AGCGCGCUuUGGGCGU- -5' |
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7722 | 3' | -56.4 | NC_001973.1 | + | 774 | 0.71 | 0.671528 |
Target: 5'- cGG-CCGUaguuGCcUCGCGUGAAAUCCGCc -3' miRNA: 3'- -UCaGGCA----CGaAGCGCGCUUUGGGCGu -5' |
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7722 | 3' | -56.4 | NC_001973.1 | + | 82249 | 0.71 | 0.671528 |
Target: 5'- uGUCCGUGUgcacgUCGCGC--AGCCCcgGCAu -3' miRNA: 3'- uCAGGCACGa----AGCGCGcuUUGGG--CGU- -5' |
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7722 | 3' | -56.4 | NC_001973.1 | + | 140700 | 0.82 | 0.172422 |
Target: 5'- cGUCCGUGUacgccaccaacugUUUGUGCGGGACCCGCGc -3' miRNA: 3'- uCAGGCACG-------------AAGCGCGCUUUGGGCGU- -5' |
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7722 | 3' | -56.4 | NC_001973.1 | + | 88897 | 1.09 | 0.002997 |
Target: 5'- cAGUCCGUGCUUCGCGCGAAACCCGCAg -3' miRNA: 3'- -UCAGGCACGAAGCGCGCUUUGGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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