Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 773 | 5' | -57.6 | NC_000852.3 | + | 272015 | 0.67 | 0.942217 |
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Target: 5'- uGAUGGUGUCa-UCAuCACCCCGgACAu -3' miRNA: 3'- -CUGCCACGGccAGUuGUGGGGUgUGU- -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 144117 | 0.67 | 0.917445 |
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Target: 5'- cGAUGGcgucgguccguuUGCUGGUUgggaacgAACACCCCAUGCu -3' miRNA: 3'- -CUGCC------------ACGGCCAG-------UUGUGGGGUGUGu -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 171045 | 0.68 | 0.88849 |
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Target: 5'- aAC-GUGCUGGUCAAgACCgCGCAUAu -3' miRNA: 3'- cUGcCACGGCCAGUUgUGGgGUGUGU- -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 211155 | 0.7 | 0.820767 |
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Target: 5'- uGGgGGuUGCCGGUCAgguaaacguccuggGCACCgUACGCGa -3' miRNA: 3'- -CUgCC-ACGGCCAGU--------------UGUGGgGUGUGU- -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 63165 | 0.72 | 0.689432 |
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Target: 5'- cGCGGUGauuCCGGUCAacaACGCCCCAUu-- -3' miRNA: 3'- cUGCCAC---GGCCAGU---UGUGGGGUGugu -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 63356 | 0.72 | 0.689432 |
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Target: 5'- cGCGGUGauuCCGGUCAacaACGCCCCAUu-- -3' miRNA: 3'- cUGCCAC---GGCCAGU---UGUGGGGUGugu -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 64112 | 0.72 | 0.689432 |
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Target: 5'- cGCGGUGauuCCGGUCAacaACGCCCCAUu-- -3' miRNA: 3'- cUGCCAC---GGCCAGU---UGUGGGGUGugu -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 64867 | 0.72 | 0.689432 |
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Target: 5'- cGCGGUGauuCCGGUCAacaACGCCCCAUu-- -3' miRNA: 3'- cUGCCAC---GGCCAGU---UGUGGGGUGugu -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 64489 | 0.72 | 0.689432 |
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Target: 5'- cGCGGUGauuCCGGUCAacaACGCCCCAUu-- -3' miRNA: 3'- cUGCCAC---GGCCAGU---UGUGGGGUGugu -5' |
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| 773 | 5' | -57.6 | NC_000852.3 | + | 87265 | 1.08 | 0.005408 |
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Target: 5'- gGACGGUGCCGGUCAACACCCCACACAg -3' miRNA: 3'- -CUGCCACGGCCAGUUGUGGGGUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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