Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 775 | 5' | -57.4 | NC_000852.3 | + | 66306 | 0.66 | 0.97156 |
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Target: 5'- uCCUuuaGAGGCUUGG--CUGCGGAUUg -3' miRNA: 3'- uGGGug-CUCCGGACCauGAUGCCUGG- -5' |
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| 775 | 5' | -57.4 | NC_000852.3 | + | 106960 | 0.67 | 0.940155 |
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Target: 5'- uGCUCGCGAGGCCgcuaaGGccgcUGCUaagacgaagcGCGaGGCCg -3' miRNA: 3'- -UGGGUGCUCCGGa----CC----AUGA----------UGC-CUGG- -5' |
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| 775 | 5' | -57.4 | NC_000852.3 | + | 178931 | 0.69 | 0.872554 |
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Target: 5'- uGCCCuCaAGGCgUGGUGCU-CGGAUa -3' miRNA: 3'- -UGGGuGcUCCGgACCAUGAuGCCUGg -5' |
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| 775 | 5' | -57.4 | NC_000852.3 | + | 321792 | 0.71 | 0.787077 |
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Target: 5'- cUCCACGAcuGGCUUGGUAacggaaaugagcUUGCGGACa -3' miRNA: 3'- uGGGUGCU--CCGGACCAU------------GAUGCCUGg -5' |
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| 775 | 5' | -57.4 | NC_000852.3 | + | 221053 | 0.72 | 0.751861 |
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Target: 5'- uACUCGCGAGGCuCUGGcucggGCUACGaACUu -3' miRNA: 3'- -UGGGUGCUCCG-GACCa----UGAUGCcUGG- -5' |
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| 775 | 5' | -57.4 | NC_000852.3 | + | 111726 | 1.1 | 0.004397 |
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Target: 5'- gACCCACGAGGCCUGGUACUACGGACCc -3' miRNA: 3'- -UGGGUGCUCCGGACCAUGAUGCCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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