Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7752 | 5' | -63.4 | NC_001973.1 | + | 56657 | 0.7 | 0.376161 |
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Target: 5'- aGGCGGGCCGUCCGugUugacguacuucaacCGCCUgUAc -3' miRNA: 3'- cCUGCCCGGCGGGCugA--------------GCGGGgAUc -5' |
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| 7752 | 5' | -63.4 | NC_001973.1 | + | 23578 | 0.7 | 0.370807 |
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Target: 5'- cGGACGcGCCGCCCGcGCUgCGCgCCa-- -3' miRNA: 3'- -CCUGCcCGGCGGGC-UGA-GCGgGGauc -5' |
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| 7752 | 5' | -63.4 | NC_001973.1 | + | 125638 | 0.71 | 0.363248 |
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Target: 5'- uGGGCGGGCCGUUCGGgUCGaggCCUGu -3' miRNA: 3'- -CCUGCCCGGCGGGCUgAGCgg-GGAUc -5' |
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| 7752 | 5' | -63.4 | NC_001973.1 | + | 120392 | 0.71 | 0.348454 |
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Target: 5'- gGGGCGauguccGCCGCCCGACgucagGCCCCa-- -3' miRNA: 3'- -CCUGCc-----CGGCGGGCUGag---CGGGGauc -5' |
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| 7752 | 5' | -63.4 | NC_001973.1 | + | 45221 | 0.73 | 0.257128 |
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Target: 5'- -uGCGGGCCGCgugcugCCGGCcCGCCaCCUGGc -3' miRNA: 3'- ccUGCCCGGCG------GGCUGaGCGG-GGAUC- -5' |
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| 7752 | 5' | -63.4 | NC_001973.1 | + | 59297 | 0.73 | 0.251416 |
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Target: 5'- cGGuCGGGCCgGCCCGcgcGCUCGCCgCa-- -3' miRNA: 3'- -CCuGCCCGG-CGGGC---UGAGCGGgGauc -5' |
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| 7752 | 5' | -63.4 | NC_001973.1 | + | 69013 | 1.1 | 0.00066 |
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Target: 5'- cGGACGGGCCGCCCGACUCGCCCCUAGa -3' miRNA: 3'- -CCUGCCCGGCGGGCUGAGCGGGGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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