Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7759 | 3' | -59.1 | NC_001973.1 | + | 22062 | 0.66 | 0.743696 |
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Target: 5'- cAUGGAcUGGGCgGCGUGUgcgGaGUGCg- -3' miRNA: 3'- uUGCCU-ACCCGaCGCACGa--C-CACGac -5' |
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| 7759 | 3' | -59.1 | NC_001973.1 | + | 81169 | 0.66 | 0.743696 |
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Target: 5'- uACGGAUcGGGCgucgggucgGCGgcgGCgacGGUGUUGg -3' miRNA: 3'- uUGCCUA-CCCGa--------CGCa--CGa--CCACGAC- -5' |
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| 7759 | 3' | -59.1 | NC_001973.1 | + | 137246 | 0.67 | 0.694308 |
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Target: 5'- gAACGGGUGGGCUuucgagccgaGUGUGCggcggcgGG-GCUc -3' miRNA: 3'- -UUGCCUACCCGA----------CGCACGa------CCaCGAc -5' |
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| 7759 | 3' | -59.1 | NC_001973.1 | + | 46338 | 0.68 | 0.633428 |
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Target: 5'- --aGGgcGcGCUGC-UGCUGGUGCUGu -3' miRNA: 3'- uugCCuaCcCGACGcACGACCACGAC- -5' |
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| 7759 | 3' | -59.1 | NC_001973.1 | + | 36145 | 0.7 | 0.503356 |
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Target: 5'- uACGGcAUGGGCaGCGcGCUGGUcGCg- -3' miRNA: 3'- uUGCC-UACCCGaCGCaCGACCA-CGac -5' |
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| 7759 | 3' | -59.1 | NC_001973.1 | + | 62467 | 1.05 | 0.002251 |
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Target: 5'- aAACGGAUGGGCUGCGUGCUGGUGCUGu -3' miRNA: 3'- -UUGCCUACCCGACGCACGACCACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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