Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
776 | 3' | -49.8 | NC_000852.3 | + | 182627 | 0.67 | 0.999596 |
Target: 5'- --aUCCCGggGAUA---ACUCGGGa- -3' miRNA: 3'- agaAGGGCuuCUAUaccUGAGCCUaa -5' |
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776 | 3' | -49.8 | NC_000852.3 | + | 109302 | 0.68 | 0.998887 |
Target: 5'- cUCgacCCCGAAGGgcAUGGAaUCGGAUUu -3' miRNA: 3'- -AGaa-GGGCUUCUa-UACCUgAGCCUAA- -5' |
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776 | 3' | -49.8 | NC_000852.3 | + | 37946 | 0.69 | 0.996836 |
Target: 5'- cUCUggCUCGggGAUAggacugGGGCUgGGGUUg -3' miRNA: 3'- -AGAa-GGGCuuCUAUa-----CCUGAgCCUAA- -5' |
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776 | 3' | -49.8 | NC_000852.3 | + | 117448 | 0.94 | 0.159245 |
Target: 5'- aUUUUcCCCGAAGAUAUGGACUCGGAUUg -3' miRNA: 3'- -AGAA-GGGCUUCUAUACCUGAGCCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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