Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
776 | 5' | -47.2 | NC_000852.3 | + | 149873 | 0.67 | 0.999993 |
Target: 5'- gGGAGAUgauAUCAgagGCCucgaaaAUCUCGGGa -3' miRNA: 3'- -UCUUUGac-UAGUa--CGGua----UAGAGCCC- -5' |
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776 | 5' | -47.2 | NC_000852.3 | + | 274014 | 0.68 | 0.999975 |
Target: 5'- uGGAACUGAgggCAcgcGCUcgGUCUCGGc -3' miRNA: 3'- uCUUUGACUa--GUa--CGGuaUAGAGCCc -5' |
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776 | 5' | -47.2 | NC_000852.3 | + | 85639 | 0.69 | 0.9999 |
Target: 5'- cAGAAGCUGccgaaUAUGCCAUGaaaauaaUCGGGg -3' miRNA: 3'- -UCUUUGACua---GUACGGUAUag-----AGCCC- -5' |
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776 | 5' | -47.2 | NC_000852.3 | + | 173770 | 0.69 | 0.999871 |
Target: 5'- aAGGAcgUGAaCGUGCCAUAUgUcCGGGa -3' miRNA: 3'- -UCUUugACUaGUACGGUAUAgA-GCCC- -5' |
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776 | 5' | -47.2 | NC_000852.3 | + | 250316 | 0.74 | 0.989573 |
Target: 5'- gGGGAACU--UCA--CCAUAUCUCGGGa -3' miRNA: 3'- -UCUUUGAcuAGUacGGUAUAGAGCCC- -5' |
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776 | 5' | -47.2 | NC_000852.3 | + | 117484 | 1.13 | 0.02556 |
Target: 5'- aAGAAACUGAUCAUGCCAUAUCUCGGGg -3' miRNA: 3'- -UCUUUGACUAGUACGGUAUAGAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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