Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7778 | 5' | -57.7 | NC_001973.1 | + | 91707 | 0.67 | 0.819555 |
Target: 5'- uGCGA--GGGUGCUCGGuCaUGGGCg- -3' miRNA: 3'- gCGCUcaCCCACGAGCU-GcACCUGaa -5' |
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7778 | 5' | -57.7 | NC_001973.1 | + | 7446 | 0.68 | 0.765931 |
Target: 5'- aCGUGAGcucgGcGGUGCggcgCGACGaGGACUUu -3' miRNA: 3'- -GCGCUCa---C-CCACGa---GCUGCaCCUGAA- -5' |
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7778 | 5' | -57.7 | NC_001973.1 | + | 74978 | 0.69 | 0.668238 |
Target: 5'- aCGgGGGgcagcGGGUGCUCGGCGuUGGugcGCUUc -3' miRNA: 3'- -GCgCUCa----CCCACGAGCUGC-ACC---UGAA- -5' |
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7778 | 5' | -57.7 | NC_001973.1 | + | 6843 | 0.73 | 0.461894 |
Target: 5'- uCGCGAGUGc--GCUCGACGgagGGGCUg -3' miRNA: 3'- -GCGCUCACccaCGAGCUGCa--CCUGAa -5' |
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7778 | 5' | -57.7 | NC_001973.1 | + | 41799 | 1.05 | 0.004056 |
Target: 5'- gCGCGAGUGGGUGCUCGACGUGGACUUc -3' miRNA: 3'- -GCGCUCACCCACGAGCUGCACCUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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