miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
778 5' -52.2 NC_000852.3 + 286203 0.66 0.999235
Target:  5'- aUUUGUCGaagcGGGu-GC-CGGGGGCUg -3'
miRNA:   3'- gGAACAGCaa--CCCuuUGaGCUCCCGA- -5'
778 5' -52.2 NC_000852.3 + 37921 0.66 0.998885
Target:  5'- --cUGggGUUGGGGguaGGCUCGGGGGa- -3'
miRNA:   3'- ggaACagCAACCCU---UUGAGCUCCCga -5'
778 5' -52.2 NC_000852.3 + 74148 0.66 0.998885
Target:  5'- ----cUCGUggUGGGAAGacgCGGGGGCa -3'
miRNA:   3'- ggaacAGCA--ACCCUUUga-GCUCCCGa -5'
778 5' -52.2 NC_000852.3 + 9181 0.69 0.99054
Target:  5'- aUUUGgaaaUGUUG-GAAACUUGAGGGCg -3'
miRNA:   3'- gGAACa---GCAACcCUUUGAGCUCCCGa -5'
778 5' -52.2 NC_000852.3 + 328688 0.69 0.989291
Target:  5'- gCUUGUCGUUGGuGAAGagguuCUUGAuGGCg -3'
miRNA:   3'- gGAACAGCAACC-CUUU-----GAGCUcCCGa -5'
778 5' -52.2 NC_000852.3 + 2056 0.69 0.989291
Target:  5'- gCUUGUCGUUGGuGAAGagguuCUUGAuGGCg -3'
miRNA:   3'- gGAACAGCAACC-CUUU-----GAGCUcCCGa -5'
778 5' -52.2 NC_000852.3 + 161696 0.97 0.084635
Target:  5'- aCCUUGUCGUUGaGGAACUCGAGGGCUg -3'
miRNA:   3'- -GGAACAGCAACcCUUUGAGCUCCCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.