Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
778 | 5' | -52.2 | NC_000852.3 | + | 286203 | 0.66 | 0.999235 |
Target: 5'- aUUUGUCGaagcGGGu-GC-CGGGGGCUg -3' miRNA: 3'- gGAACAGCaa--CCCuuUGaGCUCCCGA- -5' |
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778 | 5' | -52.2 | NC_000852.3 | + | 37921 | 0.66 | 0.998885 |
Target: 5'- --cUGggGUUGGGGguaGGCUCGGGGGa- -3' miRNA: 3'- ggaACagCAACCCU---UUGAGCUCCCga -5' |
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778 | 5' | -52.2 | NC_000852.3 | + | 74148 | 0.66 | 0.998885 |
Target: 5'- ----cUCGUggUGGGAAGacgCGGGGGCa -3' miRNA: 3'- ggaacAGCA--ACCCUUUga-GCUCCCGa -5' |
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778 | 5' | -52.2 | NC_000852.3 | + | 9181 | 0.69 | 0.99054 |
Target: 5'- aUUUGgaaaUGUUG-GAAACUUGAGGGCg -3' miRNA: 3'- gGAACa---GCAACcCUUUGAGCUCCCGa -5' |
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778 | 5' | -52.2 | NC_000852.3 | + | 328688 | 0.69 | 0.989291 |
Target: 5'- gCUUGUCGUUGGuGAAGagguuCUUGAuGGCg -3' miRNA: 3'- gGAACAGCAACC-CUUU-----GAGCUcCCGa -5' |
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778 | 5' | -52.2 | NC_000852.3 | + | 2056 | 0.69 | 0.989291 |
Target: 5'- gCUUGUCGUUGGuGAAGagguuCUUGAuGGCg -3' miRNA: 3'- gGAACAGCAACC-CUUU-----GAGCUcCCGa -5' |
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778 | 5' | -52.2 | NC_000852.3 | + | 161696 | 0.97 | 0.084635 |
Target: 5'- aCCUUGUCGUUGaGGAACUCGAGGGCUg -3' miRNA: 3'- -GGAACAGCAACcCUUUGAGCUCCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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