Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 780 | 5' | -57.1 | NC_000852.3 | + | 197346 | 0.87 | 0.119315 |
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Target: 5'- aCCGAAACCCGCUCCgaaaccugcaccgAAGCCCGCa- -3' miRNA: 3'- aGGCUUUGGGCGAGGa------------UUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197298 | 0.87 | 0.120501 |
|
Target: 5'- aCCGAAACCCGCaCCUAAGCCUGCa- -3' miRNA: 3'- aGGCUUUGGGCGaGGAUUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197465 | 0.89 | 0.091592 |
|
Target: 5'- cUCCGAAACCCGCUCCgaaaCCCGCUCc -3' miRNA: 3'- -AGGCUUUGGGCGAGGauucGGGCGAG- -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 158487 | 0.9 | 0.078728 |
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Target: 5'- aCCGAAGCCaGCUCCUAAGCCCGCa- -3' miRNA: 3'- aGGCUUUGGgCGAGGAUUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 76232 | 0.92 | 0.059503 |
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Target: 5'- cUCCuAAACCCGCUCCUAAGCCCGCa- -3' miRNA: 3'- -AGGcUUUGGGCGAGGAUUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197501 | 1.09 | 0.005262 |
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Target: 5'- cUCCGAAACCCGCUCCUAAGCCCGCUCc -3' miRNA: 3'- -AGGCUUUGGGCGAGGAUUCGGGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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