Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 780 | 5' | -57.1 | NC_000852.3 | + | 132627 | 0.67 | 0.935343 |
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Target: 5'- -gCGAAACCUGCUCCguauuauacGAGCCC-Ca- -3' miRNA: 3'- agGCUUUGGGCGAGGa--------UUCGGGcGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 185304 | 0.67 | 0.944258 |
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Target: 5'- aCCuAAGCCCuCUUCUAAGCCCuCUUc -3' miRNA: 3'- aGGcUUUGGGcGAGGAUUCGGGcGAG- -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197274 | 0.66 | 0.962898 |
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Target: 5'- gCCGAAACCCGUgCCaGuGCCUGUn- -3' miRNA: 3'- aGGCUUUGGGCGaGGaUuCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197586 | 0.66 | 0.962898 |
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Target: 5'- aCCaAAACCCGCaccaaagCCgcaccaAAGCCCGCa- -3' miRNA: 3'- aGGcUUUGGGCGa------GGa-----UUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 241705 | 0.66 | 0.968962 |
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Target: 5'- uUCUGAcgaauacaacgAGCCCGCgcccaaaCCUAAGCCCa--- -3' miRNA: 3'- -AGGCU-----------UUGGGCGa------GGAUUCGGGcgag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 283250 | 0.66 | 0.968962 |
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Target: 5'- uUCCGuugcGACCUGCUCC--AGUCCucuGUUCg -3' miRNA: 3'- -AGGCu---UUGGGCGAGGauUCGGG---CGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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