Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
780 | 5' | -57.1 | NC_000852.3 | + | 241705 | 0.66 | 0.968962 |
Target: 5'- uUCUGAcgaauacaacgAGCCCGCgcccaaaCCUAAGCCCa--- -3' miRNA: 3'- -AGGCU-----------UUGGGCGa------GGAUUCGGGcgag -5' |
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780 | 5' | -57.1 | NC_000852.3 | + | 270808 | 0.67 | 0.925571 |
Target: 5'- gUCCuAAACCCGCUCCUAAaCCa--UCa -3' miRNA: 3'- -AGGcUUUGGGCGAGGAUUcGGgcgAG- -5' |
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780 | 5' | -57.1 | NC_000852.3 | + | 270849 | 0.75 | 0.547353 |
Target: 5'- gCCcucACCgGgUCCUAAGCCCGCUCc -3' miRNA: 3'- aGGcuuUGGgCgAGGAUUCGGGCGAG- -5' |
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780 | 5' | -57.1 | NC_000852.3 | + | 273637 | 0.74 | 0.633973 |
Target: 5'- aCCGGGACCUGCUCCUGuGCCgagaccgagCGCg- -3' miRNA: 3'- aGGCUUUGGGCGAGGAUuCGG---------GCGag -5' |
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780 | 5' | -57.1 | NC_000852.3 | + | 283250 | 0.66 | 0.968962 |
Target: 5'- uUCCGuugcGACCUGCUCC--AGUCCucuGUUCg -3' miRNA: 3'- -AGGCu---UUGGGCGAGGauUCGGG---CGAG- -5' |
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780 | 5' | -57.1 | NC_000852.3 | + | 329362 | 0.69 | 0.856809 |
Target: 5'- cUuuGGGcUCCGCUCCUGgucgaacaugGGCuCCGCUCc -3' miRNA: 3'- -AggCUUuGGGCGAGGAU----------UCG-GGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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