Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 780 | 5' | -57.1 | NC_000852.3 | + | 122627 | 0.79 | 0.36587 |
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Target: 5'- gCCGAAGCCUGCaCCgAAGCCCGCg- -3' miRNA: 3'- aGGCUUUGGGCGaGGaUUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 76173 | 0.79 | 0.358305 |
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Target: 5'- aCCcAAACCCGCUCCUAAaCCCGCa- -3' miRNA: 3'- aGGcUUUGGGCGAGGAUUcGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 24308 | 0.79 | 0.350851 |
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Target: 5'- gCCGAAACCUGCUCCcAAaCCUGCUCc -3' miRNA: 3'- aGGCUUUGGGCGAGGaUUcGGGCGAG- -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197430 | 0.8 | 0.343507 |
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Target: 5'- aCCGAAACCUGCaCCgAAGCCCGCa- -3' miRNA: 3'- aGGCUUUGGGCGaGGaUUCGGGCGag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 158331 | 0.8 | 0.343507 |
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Target: 5'- aCCGAAACCCGCUCCUAAaCCCa--- -3' miRNA: 3'- aGGCUUUGGGCGAGGAUUcGGGcgag -5' |
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| 780 | 5' | -57.1 | NC_000852.3 | + | 197501 | 1.09 | 0.005262 |
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Target: 5'- cUCCGAAACCCGCUCCUAAGCCCGCUCc -3' miRNA: 3'- -AGGCUUUGGGCGAGGAUUCGGGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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