Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 783 | 3' | -44.2 | NC_000852.3 | + | 270424 | 0.67 | 1 |
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Target: 5'- cUCGguccaGggGAUGGUuuagGACCCGGu -3' miRNA: 3'- aAGCag---CaaUUAUCAuuaaCUGGGCC- -5' |
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| 783 | 3' | -44.2 | NC_000852.3 | + | 2380 | 0.66 | 1 |
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Target: 5'- -aCGUCGgaGAUucAGggug-GACCCGGu -3' miRNA: 3'- aaGCAGCaaUUA--UCauuaaCUGGGCC- -5' |
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| 783 | 3' | -44.2 | NC_000852.3 | + | 253185 | 0.71 | 0.999996 |
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Target: 5'- -aUGUCGUUuGUgccGGUAAUUGugUCGGu -3' miRNA: 3'- aaGCAGCAAuUA---UCAUUAACugGGCC- -5' |
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| 783 | 3' | -44.2 | NC_000852.3 | + | 119433 | 0.71 | 0.999994 |
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Target: 5'- -cUGUCGcggUAggAGUGAaUGGCCCGGc -3' miRNA: 3'- aaGCAGCa--AUuaUCAUUaACUGGGCC- -5' |
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| 783 | 3' | -44.2 | NC_000852.3 | + | 45270 | 0.72 | 0.999971 |
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Target: 5'- -cCGUCGUaaugaugaUAAUAGU-AUUG-CCCGGg -3' miRNA: 3'- aaGCAGCA--------AUUAUCAuUAACuGGGCC- -5' |
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| 783 | 3' | -44.2 | NC_000852.3 | + | 257228 | 1.1 | 0.081169 |
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Target: 5'- aUUCGUCGUUAAUAGUAAUUGACCCGGu -3' miRNA: 3'- -AAGCAGCAAUUAUCAUUAACUGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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