Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7836 | 5' | -52.8 | NC_001973.1 | + | 28604 | 0.66 | 0.981268 |
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Target: 5'- uGGCCAGGAucgaauugauguuGGUGagGUCGUUGcGCa -3' miRNA: 3'- gCUGGUCUU-------------CCACagCAGCAACcUGc -5' |
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| 7836 | 5' | -52.8 | NC_001973.1 | + | 103919 | 0.67 | 0.96887 |
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Target: 5'- gCGACgAGGAGcGcGUCGUCGcggcgcgGGGCGu -3' miRNA: 3'- -GCUGgUCUUC-CaCAGCAGCaa-----CCUGC- -5' |
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| 7836 | 5' | -52.8 | NC_001973.1 | + | 74970 | 0.7 | 0.910608 |
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Target: 5'- aGGCCAGAacggggggcagcGGGUGcUCGgCGUUGGuGCGc -3' miRNA: 3'- gCUGGUCU------------UCCAC-AGCaGCAACC-UGC- -5' |
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| 7836 | 5' | -52.8 | NC_001973.1 | + | 117534 | 0.71 | 0.847232 |
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Target: 5'- aCGGCgGuGggGGUGUCGUCcgacgcGGACGa -3' miRNA: 3'- -GCUGgU-CuuCCACAGCAGcaa---CCUGC- -5' |
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| 7836 | 5' | -52.8 | NC_001973.1 | + | 94913 | 0.73 | 0.785921 |
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Target: 5'- cCGACCGGcAAGGUGguggccgccuacuUCGUCGagugGGGCGu -3' miRNA: 3'- -GCUGGUC-UUCCAC-------------AGCAGCaa--CCUGC- -5' |
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| 7836 | 5' | -52.8 | NC_001973.1 | + | 4985 | 0.98 | 0.037195 |
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Target: 5'- gCGACC-GAAGGUGUCGUCGUUGGACGc -3' miRNA: 3'- -GCUGGuCUUCCACAGCAGCAACCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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