Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 784 | 5' | -48.8 | NC_000852.3 | + | 211211 | 0.65 | 0.999985 |
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Target: 5'- uCCUCCGgccauauCGAGUuauUGAUACUAUUAc -3' miRNA: 3'- cGGAGGC-------GCUCAuu-GCUGUGAUAAUu -5' |
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| 784 | 5' | -48.8 | NC_000852.3 | + | 88521 | 0.66 | 0.999981 |
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Target: 5'- cGCCU--GCGAGUuuAugGACGgUGUUGAg -3' miRNA: 3'- -CGGAggCGCUCA--UugCUGUgAUAAUU- -5' |
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| 784 | 5' | -48.8 | NC_000852.3 | + | 166747 | 0.66 | 0.999974 |
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Target: 5'- aGCUUCCGCGAuGUcacAACGACcACa----- -3' miRNA: 3'- -CGGAGGCGCU-CA---UUGCUG-UGauaauu -5' |
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| 784 | 5' | -48.8 | NC_000852.3 | + | 125730 | 0.67 | 0.999896 |
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Target: 5'- -aCUUCG-GAGUAACGACACa----- -3' miRNA: 3'- cgGAGGCgCUCAUUGCUGUGauaauu -5' |
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| 784 | 5' | -48.8 | NC_000852.3 | + | 286824 | 0.68 | 0.999655 |
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Target: 5'- uGCCUCUGCGuG-AACGACGuaAUUAu -3' miRNA: 3'- -CGGAGGCGCuCaUUGCUGUgaUAAUu -5' |
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| 784 | 5' | -48.8 | NC_000852.3 | + | 282800 | 1.09 | 0.03081 |
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Target: 5'- uGCCUCCGCGAGUAACGACACUAUUAAc -3' miRNA: 3'- -CGGAGGCGCUCAUUGCUGUGAUAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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