miRNA: 3'- -CCuaUCCCCU-GC-GCGaaCUUGCGCGA- -5'
miRNA: 3'- cCUAU-----CCcCUGCGCGAACUUGCGCGA- -5'
miRNA: 3'- cCUAU--CCCCugCGCGaACU-----UGCGCGa -5'
miRNA: 3'- -CCUAUCCCCUGCGCGAACUUGCGCGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7841 | 3' | -55.4 | NC_001973.1 | + | 111248 | 0.72 | 0.631504 |
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Target: 5'- gGGcgAGGGGGuCGaCGC--GGACGCGCUg -3' miRNA: 3'- -CCuaUCCCCU-GC-GCGaaCUUGCGCGA- -5' |
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| 7841 | 3' | -55.4 | NC_001973.1 | + | 80688 | 0.73 | 0.590765 |
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Target: 5'- aGAUGcuguuGGcGAUGCGCUUGAGCGCGUUc -3' miRNA: 3'- cCUAU-----CCcCUGCGCGAACUUGCGCGA- -5' |
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| 7841 | 3' | -55.4 | NC_001973.1 | + | 101548 | 0.74 | 0.570547 |
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Target: 5'- uGAUGuuGGGGugGCGCgUGGuccagGCGCGCa -3' miRNA: 3'- cCUAU--CCCCugCGCGaACU-----UGCGCGa -5' |
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| 7841 | 3' | -55.4 | NC_001973.1 | + | 3999 | 1.11 | 0.002782 |
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Target: 5'- cGGAUAGGGGACGCGCUUGAACGCGCUg -3' miRNA: 3'- -CCUAUCCCCUGCGCGAACUUGCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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