Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 785 | 3' | -54.2 | NC_000852.3 | + | 54632 | 0.66 | 0.993683 |
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Target: 5'- uUGACGAcgugaAGGCGUu-CCUggGGCUCGa -3' miRNA: 3'- -ACUGCU-----UCCGCAccGGAuaCUGAGCc -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 256819 | 0.66 | 0.992785 |
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Target: 5'- uUGuuCGGAGaGCGUGuuuuaGCCUAUGACacUUGGa -3' miRNA: 3'- -ACu-GCUUC-CGCAC-----CGGAUACUG--AGCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 243360 | 0.66 | 0.991789 |
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Target: 5'- aGACGGAGGUaaUGGUCUAUa--UCGGa -3' miRNA: 3'- aCUGCUUCCGc-ACCGGAUAcugAGCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 210493 | 0.67 | 0.990686 |
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Target: 5'- gUGGCgGAGGGgGUGGCg---GuCUCGGa -3' miRNA: 3'- -ACUG-CUUCCgCACCGgauaCuGAGCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 37871 | 0.67 | 0.988135 |
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Target: 5'- nGGgGAGGGCucggGGCUggggGUaGGCUCGGg -3' miRNA: 3'- aCUgCUUCCGca--CCGGa---UA-CUGAGCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 37901 | 0.67 | 0.988135 |
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Target: 5'- gGGgGAGGGCucggGGCUggggGUaGGCUCGGg -3' miRNA: 3'- aCUgCUUCCGca--CCGGa---UA-CUGAGCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 168070 | 0.69 | 0.969515 |
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Target: 5'- cGGgGGAGGagGUGGaCUUGUGACacUCGGg -3' miRNA: 3'- aCUgCUUCCg-CACC-GGAUACUG--AGCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 93350 | 0.7 | 0.935926 |
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Target: 5'- aUGAgGggGGUuUGGUCUAgGACUgGGg -3' miRNA: 3'- -ACUgCuuCCGcACCGGAUaCUGAgCC- -5' |
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| 785 | 3' | -54.2 | NC_000852.3 | + | 285713 | 1.1 | 0.008542 |
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Target: 5'- uUGACGAAGGCGUGGCCUAUGACUCGGu -3' miRNA: 3'- -ACUGCUUCCGCACCGGAUACUGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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