Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 785 | 5' | -53.4 | NC_000852.3 | + | 328591 | 0.66 | 0.99649 |
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Target: 5'- gGCCGUcauUUCCAUucGUCAUUuccauUCGUCa -3' miRNA: 3'- gCGGCA---AAGGUGuuCGGUAGu----GGCAG- -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 2153 | 0.66 | 0.99649 |
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Target: 5'- gGCCGUcauUUCCAUucGUCAUUuccauUCGUCa -3' miRNA: 3'- gCGGCA---AAGGUGuuCGGUAGu----GGCAG- -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 264922 | 0.66 | 0.995921 |
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Target: 5'- uCGCCGUUUCUACAGGUauuUCuauuCgCGUUc -3' miRNA: 3'- -GCGGCAAAGGUGUUCGgu-AGu---G-GCAG- -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 3403 | 0.67 | 0.991838 |
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Target: 5'- uCGCCGccUCUGauuGGCCAUCGCgaGUCa -3' miRNA: 3'- -GCGGCaaAGGUgu-UCGGUAGUGg-CAG- -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 161994 | 0.67 | 0.990725 |
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Target: 5'- uGCUGUa--UAgGucGCCAUCACCGUCa -3' miRNA: 3'- gCGGCAaagGUgUu-CGGUAGUGGCAG- -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 94321 | 0.67 | 0.988142 |
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Target: 5'- gCGCCGUgaccUCACAcucgcccuGGCUGUUGCCGUUg -3' miRNA: 3'- -GCGGCAaa--GGUGU--------UCGGUAGUGGCAG- -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 86081 | 0.68 | 0.97838 |
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Target: 5'- gGCCGUUauuccgucaggcgaUgCCGCAAGCCAuggguacUCAUCGUg -3' miRNA: 3'- gCGGCAA--------------A-GGUGUUCGGU-------AGUGGCAg -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 120040 | 0.69 | 0.971975 |
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Target: 5'- -uUUGUaUCCGCAuguGCCAUCACCGa- -3' miRNA: 3'- gcGGCAaAGGUGUu--CGGUAGUGGCag -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 149464 | 0.73 | 0.868627 |
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Target: 5'- cCGCCGUUUgCCAUucuuuGUCGUCGCCGa- -3' miRNA: 3'- -GCGGCAAA-GGUGuu---CGGUAGUGGCag -5' |
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| 785 | 5' | -53.4 | NC_000852.3 | + | 285747 | 1.09 | 0.011117 |
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Target: 5'- gCGCCGUUUCCACAAGCCAUCACCGUCc -3' miRNA: 3'- -GCGGCAAAGGUGUUCGGUAGUGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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