Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
786 | 3' | -58 | NC_000852.3 | + | 122635 | 0.66 | 0.957836 |
Target: 5'- cUGCaCCGAaGcCCGCGCCgaaacccgcGCCGAAAc -3' miRNA: 3'- uGCG-GGCUaCuGGCGCGG---------UGGCUUUc -5' |
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786 | 3' | -58 | NC_000852.3 | + | 213787 | 0.66 | 0.94236 |
Target: 5'- cGCGgaaCUCGcgGuCgGCGCCACCGAGGa -3' miRNA: 3'- -UGC---GGGCuaCuGgCGCGGUGGCUUUc -5' |
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786 | 3' | -58 | NC_000852.3 | + | 96294 | 0.67 | 0.937531 |
Target: 5'- uGCGCCCgGAUGAUCGauggguacagacuCGCaaaAUCGAGAGc -3' miRNA: 3'- -UGCGGG-CUACUGGC-------------GCGg--UGGCUUUC- -5' |
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786 | 3' | -58 | NC_000852.3 | + | 133386 | 0.67 | 0.9286 |
Target: 5'- --aCUCGGUaACCGaCGCCGCCGAAAc -3' miRNA: 3'- ugcGGGCUAcUGGC-GCGGUGGCUUUc -5' |
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786 | 3' | -58 | NC_000852.3 | + | 271604 | 0.68 | 0.876175 |
Target: 5'- aGCGUCCGGUGGCauaaGCCGCUGGu-- -3' miRNA: 3'- -UGCGGGCUACUGgcg-CGGUGGCUuuc -5' |
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786 | 3' | -58 | NC_000852.3 | + | 309877 | 1.07 | 0.006164 |
Target: 5'- cACGCCCGAUGACCGCGCCACCGAAAGc -3' miRNA: 3'- -UGCGGGCUACUGGCGCGGUGGCUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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