Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7865 | 3' | -57.2 | NC_001973.1 | + | 158799 | 0.68 | 0.744507 |
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Target: 5'- -aUAGAGCGggUcgcccuGCUUGGCCaaGGCg -3' miRNA: 3'- uaGUCUCGCuuG------CGAACUGGggCCGg -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 119989 | 0.69 | 0.725077 |
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Target: 5'- cAUCGG-GCuuGAACcCgaucGACCCCGGCCg -3' miRNA: 3'- -UAGUCuCG--CUUGcGaa--CUGGGGCCGG- -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 104975 | 0.69 | 0.725077 |
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Target: 5'- cUCGGAGCcuGAGCGCUcUGAgCCuucgcucacauCGGCCu -3' miRNA: 3'- uAGUCUCG--CUUGCGA-ACUgGG-----------GCCGG- -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 9859 | 0.69 | 0.715239 |
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Target: 5'- gGUCAGAGCcGAUGacaucauGCCUCGGCCg -3' miRNA: 3'- -UAGUCUCGcUUGCgaac---UGGGGCCGG- -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 110270 | 0.69 | 0.704337 |
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Target: 5'- uGUUGGAGCGGgccagcaACGCauaGACgCCCGGCUc -3' miRNA: 3'- -UAGUCUCGCU-------UGCGaa-CUG-GGGCCGG- -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 132636 | 0.69 | 0.685342 |
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Target: 5'- cGUCAguGAGC--GCGCUUGgcgGCCgCGGCCg -3' miRNA: 3'- -UAGU--CUCGcuUGCGAAC---UGGgGCCGG- -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 39829 | 0.7 | 0.655055 |
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Target: 5'- gGUCAGAGCGAGCGCcacgcGGCggCgCGGCg -3' miRNA: 3'- -UAGUCUCGCUUGCGaa---CUG--GgGCCGg -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 115187 | 0.7 | 0.655055 |
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Target: 5'- cGUCGcGGGCGcGCGCU---CCUCGGCCg -3' miRNA: 3'- -UAGU-CUCGCuUGCGAacuGGGGCCGG- -5' |
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| 7865 | 3' | -57.2 | NC_001973.1 | + | 34949 | 0.77 | 0.297287 |
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Target: 5'- --gAGAGCGugccGACGCgUGAgCCCGGCCg -3' miRNA: 3'- uagUCUCGC----UUGCGaACUgGGGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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