Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
787 | 5' | -51 | NC_000852.3 | + | 159550 | 0.66 | 0.999653 |
Target: 5'- aUCGUCUACCA---UUGUUagGUUCCg -3' miRNA: 3'- cAGCAGGUGGUcaaAGCGGuaCAAGG- -5' |
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787 | 5' | -51 | NC_000852.3 | + | 159473 | 0.66 | 0.999653 |
Target: 5'- ---uUCCACauaaaaGGUUUCGCauuUGUUCCu -3' miRNA: 3'- cagcAGGUGg-----UCAAAGCGgu-ACAAGG- -5' |
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787 | 5' | -51 | NC_000852.3 | + | 138373 | 0.68 | 0.998035 |
Target: 5'- uUCGUUaauaaaAUCGGUUUCGUCGUGgugCCu -3' miRNA: 3'- cAGCAGg-----UGGUCAAAGCGGUACaa-GG- -5' |
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787 | 5' | -51 | NC_000852.3 | + | 313444 | 0.73 | 0.957237 |
Target: 5'- aGUCGUCCACCAGUuccUUCGCaaacuuugGUUg- -3' miRNA: 3'- -CAGCAGGUGGUCA---AAGCGgua-----CAAgg -5' |
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787 | 5' | -51 | NC_000852.3 | + | 320006 | 1.12 | 0.012701 |
Target: 5'- gGUCGUCCACCAGUUUCGCCAUGUUCCu -3' miRNA: 3'- -CAGCAGGUGGUCAAAGCGGUACAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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