miRNA: 3'- -AGGACAGGGCAACUCGGAGUUGCGGG- -5'
miRNA: 3'- aGGACAGGG-CAACUcGG----AGUugCGGg -5'
miRNA: 3'- -AGGACAGGGcaACuCGGaGUUGCGGG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 790 | 5' | -56.4 | NC_000855.1 | + | 844 | 1.11 | 5.4e-05 |
|
Target: 5'- uUCCUGUCCCGUUGAGCCUCAACGCCCc -3' miRNA: 3'- -AGGACAGGGCAACUCGGAGUUGCGGG- -5' |
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| 790 | 5' | -56.4 | NC_000855.1 | + | 6210 | 0.66 | 0.246014 |
|
Target: 5'- cCCUGUCCUaGUUGAcCCagauUCAugGCUg -3' miRNA: 3'- aGGACAGGG-CAACUcGG----AGUugCGGg -5' |
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| 790 | 5' | -56.4 | NC_000855.1 | + | 8630 | 0.66 | 0.238999 |
|
Target: 5'- cUCUUGUCCC--UGuGUUcCAAUGCCCu -3' miRNA: 3'- -AGGACAGGGcaACuCGGaGUUGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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