Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7927 | 3' | -52.5 | NC_001973.1 | + | 95329 | 0.66 | 0.978043 |
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Target: 5'- cGUCGAGuCGGCg--CGCGaguuUCUGCAGAc -3' miRNA: 3'- -CAGCUU-GUCGaagGUGC----AGACGUCUu -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 95072 | 0.66 | 0.969852 |
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Target: 5'- -gCGGGCAGCUUCgagGCG-CUGCAGc- -3' miRNA: 3'- caGCUUGUCGAAGg--UGCaGACGUCuu -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 39072 | 0.66 | 0.969852 |
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Target: 5'- gGUCGGugGGCggCauuGCGUCaGCGGAAa -3' miRNA: 3'- -CAGCUugUCGaaGg--UGCAGaCGUCUU- -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 83806 | 0.66 | 0.969852 |
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Target: 5'- cUCGGACgAGCUgggCCGCG-CgcgGCGGAGc -3' miRNA: 3'- cAGCUUG-UCGAa--GGUGCaGa--CGUCUU- -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 84295 | 0.67 | 0.963302 |
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Target: 5'- cUCGAACGGCgcacggCCGCGUCcGCcgcccAGGAc -3' miRNA: 3'- cAGCUUGUCGaa----GGUGCAGaCG-----UCUU- -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 89063 | 0.67 | 0.95582 |
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Target: 5'- -aCGGGCAGCcggUCCAUGU--GCAGAGu -3' miRNA: 3'- caGCUUGUCGa--AGGUGCAgaCGUCUU- -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 137210 | 0.68 | 0.937901 |
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Target: 5'- aGUCGcGCGGCgg-CGCGUCcGCAGAc -3' miRNA: 3'- -CAGCuUGUCGaagGUGCAGaCGUCUu -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 39712 | 0.69 | 0.882666 |
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Target: 5'- cGUCGAGCAGCgcggccgCCAUGUCcGCGu-- -3' miRNA: 3'- -CAGCUUGUCGaa-----GGUGCAGaCGUcuu -5' |
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| 7927 | 3' | -52.5 | NC_001973.1 | + | 70135 | 0.71 | 0.808358 |
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Target: 5'- uGUCGAgcauggugcGCAGCgcgUCCGCGUCcGCGGc- -3' miRNA: 3'- -CAGCU---------UGUCGa--AGGUGCAGaCGUCuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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