Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7929 | 5' | -57.5 | NC_001973.1 | + | 62452 | 0.66 | 0.85024 |
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Target: 5'- aGCCUacUGGAGCUGaaaCG-GAUGGGCUg -3' miRNA: 3'- -CGGA--GCUUCGACgg-GCgCUACCUGAa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 51814 | 0.66 | 0.85024 |
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Target: 5'- uGCUgcgCGAGGC-GCCCGCGugcGUGGuGCg- -3' miRNA: 3'- -CGGa--GCUUCGaCGGGCGC---UACC-UGaa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 29951 | 0.66 | 0.841451 |
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Target: 5'- cGCCUCGAGcccguacagagacGCcgGCUCgGUGGUGGGCg- -3' miRNA: 3'- -CGGAGCUU-------------CGa-CGGG-CGCUACCUGaa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 46100 | 0.66 | 0.825738 |
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Target: 5'- cGCUaCGGccGCgUGCCCGCGcUGGACg- -3' miRNA: 3'- -CGGaGCUu-CG-ACGGGCGCuACCUGaa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 9931 | 0.67 | 0.781516 |
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Target: 5'- uGCCgcggGAAGUUGCCCGCcgcgacGAcGGACUc -3' miRNA: 3'- -CGGag--CUUCGACGGGCG------CUaCCUGAa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 135673 | 0.67 | 0.781516 |
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Target: 5'- gGCCgUGgcGCUgGCCUGCGGuuUGGACg- -3' miRNA: 3'- -CGGaGCuuCGA-CGGGCGCU--ACCUGaa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 38392 | 0.67 | 0.772238 |
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Target: 5'- cGCCaCGAAGCUGUCgaCGUGAUGGuCc- -3' miRNA: 3'- -CGGaGCUUCGACGG--GCGCUACCuGaa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 64883 | 0.68 | 0.733975 |
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Target: 5'- cGCC-CGAAG-UGCUgGCGcgGGGCUUu -3' miRNA: 3'- -CGGaGCUUCgACGGgCGCuaCCUGAA- -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 40342 | 0.69 | 0.694308 |
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Target: 5'- cGCUUCGuAGUUGUCCGCGGUG-ACc- -3' miRNA: 3'- -CGGAGCuUCGACGGGCGCUACcUGaa -5' |
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| 7929 | 5' | -57.5 | NC_001973.1 | + | 35504 | 0.74 | 0.420354 |
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Target: 5'- cGCCUCGAccccgAGCUcGCCCGcCGAcUGGugUa -3' miRNA: 3'- -CGGAGCU-----UCGA-CGGGC-GCU-ACCugAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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