Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
796 | 5' | -51.2 | NC_000855.1 | + | 479 | 0.66 | 0.455991 |
Target: 5'- cGAGGCGGGAAucauGAUgCCCAugAg-- -3' miRNA: 3'- cCUUCGUCCUUu---CUAgGGGUugUggu -5' |
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796 | 5' | -51.2 | NC_000855.1 | + | 6437 | 0.66 | 0.422131 |
Target: 5'- aGGAgaaugaagacAGCGuGGAAAagguGAUCCUCAAgGCCGa -3' miRNA: 3'- -CCU----------UCGU-CCUUU----CUAGGGGUUgUGGU- -5' |
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796 | 5' | -51.2 | NC_000855.1 | + | 2000 | 0.68 | 0.349355 |
Target: 5'- -cAAGCuGG-AAGAUCCCCuuGCACUc -3' miRNA: 3'- ccUUCGuCCuUUCUAGGGGu-UGUGGu -5' |
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796 | 5' | -51.2 | NC_000855.1 | + | 4938 | 0.69 | 0.311965 |
Target: 5'- gGGAGGUucAGGAGGGGagCCCAGaGCCu -3' miRNA: 3'- -CCUUCG--UCCUUUCUagGGGUUgUGGu -5' |
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796 | 5' | -51.2 | NC_000855.1 | + | 7726 | 0.73 | 0.159557 |
Target: 5'- uGGggGUGGGAAAGGggagcaUCCCCAGaGCUg -3' miRNA: 3'- -CCuuCGUCCUUUCU------AGGGGUUgUGGu -5' |
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796 | 5' | -51.2 | NC_000855.1 | + | 6221 | 1.12 | 0.000176 |
Target: 5'- uGGAAGCAGGAAAGAUCCCCAACACCAa -3' miRNA: 3'- -CCUUCGUCCUUUCUAGGGGUUGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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