Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7977 | 3' | -57.4 | NC_001973.1 | + | 117220 | 0.7 | 0.648977 |
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Target: 5'- --cGCgGCGGCUUGGGCuuccuuaUGA-CCAGCu -3' miRNA: 3'- ugaCGaCGCCGAGCUCG-------ACUaGGUCG- -5' |
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| 7977 | 3' | -57.4 | NC_001973.1 | + | 140016 | 0.7 | 0.629577 |
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Target: 5'- uACUGCUGCcagagcacGGUggCGGGCUGcgCCcgGGCg -3' miRNA: 3'- -UGACGACG--------CCGa-GCUCGACuaGG--UCG- -5' |
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| 7977 | 3' | -57.4 | NC_001973.1 | + | 115530 | 0.71 | 0.588822 |
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Target: 5'- uGCUGUgggagcGCGGCUaCGAgaaccacuacacGCUGggCCAGCa -3' miRNA: 3'- -UGACGa-----CGCCGA-GCU------------CGACuaGGUCG- -5' |
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| 7977 | 3' | -57.4 | NC_001973.1 | + | 61192 | 0.72 | 0.499546 |
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Target: 5'- cGCUGaacGCGcGCUCGGGCggGcgCCGGCg -3' miRNA: 3'- -UGACga-CGC-CGAGCUCGa-CuaGGUCG- -5' |
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| 7977 | 3' | -57.4 | NC_001973.1 | + | 33968 | 0.72 | 0.48997 |
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Target: 5'- aGCcGCUGCGGCgucaugaucagCGAGUUGAUgCGGUu -3' miRNA: 3'- -UGaCGACGCCGa----------GCUCGACUAgGUCG- -5' |
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| 7977 | 3' | -57.4 | NC_001973.1 | + | 157880 | 0.73 | 0.461777 |
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Target: 5'- --cGCUGCGGCUCGugucGgaGGUCgCGGCc -3' miRNA: 3'- ugaCGACGCCGAGCu---CgaCUAG-GUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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