Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7984 | 3' | -60.5 | NC_001973.1 | + | 109576 | 0.67 | 0.679051 |
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Target: 5'- -gCGGGUUUCGGGCGCgagCC-GUCCAc -3' miRNA: 3'- gaGUCCGAGGCUCGCGag-GGaCAGGU- -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 133902 | 0.68 | 0.619476 |
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Target: 5'- uCUCGGcGC-CCucGCGCgucgccgccgCCCUGUCCAg -3' miRNA: 3'- -GAGUC-CGaGGcuCGCGa---------GGGACAGGU- -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 158689 | 0.68 | 0.615499 |
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Target: 5'- ---cGGCggCCGAGCGCgcgccuaccgacgCCCUGUUCGc -3' miRNA: 3'- gaguCCGa-GGCUCGCGa------------GGGACAGGU- -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 52812 | 0.68 | 0.609536 |
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Target: 5'- aCUCGGGCU-CGAGCGCg--CUGUCg- -3' miRNA: 3'- -GAGUCCGAgGCUCGCGaggGACAGgu -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 61204 | 0.68 | 0.589711 |
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Target: 5'- gCUCGGGCgggcgCCG-GCGCUCC---UCCAa -3' miRNA: 3'- -GAGUCCGa----GGCuCGCGAGGgacAGGU- -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 47764 | 0.68 | 0.570002 |
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Target: 5'- aUCAcGGcCUCCucGGCGCUCCUcagGUCCAg -3' miRNA: 3'- gAGU-CC-GAGGc-UCGCGAGGGa--CAGGU- -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 144203 | 0.69 | 0.550458 |
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Target: 5'- -gCAGGCUCuCGAGCGCggcgCCgaGgCCGa -3' miRNA: 3'- gaGUCCGAG-GCUCGCGa---GGgaCaGGU- -5' |
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| 7984 | 3' | -60.5 | NC_001973.1 | + | 111960 | 0.69 | 0.540762 |
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Target: 5'- ---cGGCggCCGAGCGCgugCCCaUGUUCAa -3' miRNA: 3'- gaguCCGa-GGCUCGCGa--GGG-ACAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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