miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7992 3' -54.1 NC_001973.1 + 51334 0.66 0.960939
Target:  5'- cGGAguugUAGGUGGCgccacaaguugGUCCGGuCUgcGGACCa -3'
miRNA:   3'- -CCU----GUUCACCGa----------CAGGUC-GA--UCUGGc -5'
7992 3' -54.1 NC_001973.1 + 95755 0.66 0.956893
Target:  5'- cGACAAgaucgugcucggcGUGGCcaUGUacgggcgCGGCUGGACCGg -3'
miRNA:   3'- cCUGUU-------------CACCG--ACAg------GUCGAUCUGGC- -5'
7992 3' -54.1 NC_001973.1 + 6483 0.67 0.930354
Target:  5'- aGGACGucaAGggcGuGCUGUaCCGGCUGGGCgGc -3'
miRNA:   3'- -CCUGU---UCa--C-CGACA-GGUCGAUCUGgC- -5'
7992 3' -54.1 NC_001973.1 + 104367 0.68 0.913639
Target:  5'- cGAuCAAGcgGGCguccucGUCCAGCUcGACCa -3'
miRNA:   3'- cCU-GUUCa-CCGa-----CAGGUCGAuCUGGc -5'
7992 3' -54.1 NC_001973.1 + 81077 0.68 0.901288
Target:  5'- gGGACAGGUcgauGGC-GUUCGcGCUGGGCgCGg -3'
miRNA:   3'- -CCUGUUCA----CCGaCAGGU-CGAUCUG-GC- -5'
7992 3' -54.1 NC_001973.1 + 151017 0.7 0.842755
Target:  5'- uGAUu-GUGccuGCUGUCCGGCUcGGCCGa -3'
miRNA:   3'- cCUGuuCAC---CGACAGGUCGAuCUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.