miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
80 5' -59.8 AC_000005.1 + 14777 1.09 0.000195
Target:  5'- aGUCACCAUCACUGACGCCCGCCGCCGg -3'
miRNA:   3'- -CAGUGGUAGUGACUGCGGGCGGCGGC- -5'
80 5' -59.8 AC_000005.1 + 1930 0.7 0.185719
Target:  5'- gGUUugCAuuuacUCGCUGGCGCggccuuugaccaugCCGCCGCUGc -3'
miRNA:   3'- -CAGugGU-----AGUGACUGCG--------------GGCGGCGGC- -5'
80 5' -59.8 AC_000005.1 + 15678 0.68 0.270695
Target:  5'- -aCGCCGUCuGCUGACGCC-GCgGCg- -3'
miRNA:   3'- caGUGGUAG-UGACUGCGGgCGgCGgc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.