Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8029 | 3' | -56.4 | NC_001973.1 | + | 145034 | 0.68 | 0.800719 |
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Target: 5'- uGCGGCGCGAgCGcgUCGAGCgCCgACUc- -3' miRNA: 3'- -CGCCGCGCU-GCa-GGUUCG-GGaUGAaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 61124 | 0.68 | 0.809584 |
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Target: 5'- cGgGGCGCGGCGUCgcuccuCGAGCUCgUACc-- -3' miRNA: 3'- -CgCCGCGCUGCAG------GUUCGGG-AUGaaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 45967 | 0.68 | 0.809584 |
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Target: 5'- gGCGGC-CGGCG-CgCucGCCCUGCUg- -3' miRNA: 3'- -CGCCGcGCUGCaG-GuuCGGGAUGAaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 97994 | 0.68 | 0.809584 |
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Target: 5'- cGCGGUcgGCGACGUCCAcGCgCUcCg-- -3' miRNA: 3'- -CGCCG--CGCUGCAGGUuCGgGAuGaaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 39429 | 0.67 | 0.826824 |
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Target: 5'- -aGGCGCGucGCGUCCAGggcgacgccgauGCCCaGCUc- -3' miRNA: 3'- cgCCGCGC--UGCAGGUU------------CGGGaUGAaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 53859 | 0.67 | 0.826824 |
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Target: 5'- gGCGGCgGCGGCGccgCCGAGCgCgGCg-- -3' miRNA: 3'- -CGCCG-CGCUGCa--GGUUCGgGaUGaaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 1580 | 0.67 | 0.827668 |
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Target: 5'- cUGGCGCGACGaauugaucgccuccaCCAGGUCCUcCUUg -3' miRNA: 3'- cGCCGCGCUGCa--------------GGUUCGGGAuGAAa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 33132 | 0.67 | 0.835182 |
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Target: 5'- -aGGCGCuGCGUCgc-GCCCUGCa-- -3' miRNA: 3'- cgCCGCGcUGCAGguuCGGGAUGaaa -5' |
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| 8029 | 3' | -56.4 | NC_001973.1 | + | 105883 | 0.66 | 0.901323 |
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Target: 5'- gGUGGCGaCGACG-CCGAGCUg-GCg-- -3' miRNA: 3'- -CGCCGC-GCUGCaGGUUCGGgaUGaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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