Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8035 | 3' | -55 | NC_001973.1 | + | 27525 | 0.66 | 0.924051 |
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Target: 5'- gGAACA-GUUGggcggcuuguAGCAGUCCUCcCGUCUg -3' miRNA: 3'- -CUUGUgCAAC----------UCGUCAGGGGcGCAGA- -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 13313 | 0.67 | 0.918456 |
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Target: 5'- gGAACGCGcgGuGCAGUaCCCgGCGg-- -3' miRNA: 3'- -CUUGUGCaaCuCGUCA-GGGgCGCaga -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 143004 | 0.68 | 0.872677 |
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Target: 5'- uGAACACGcaccUGaAGCAGcugugCCCCGCGggCUa -3' miRNA: 3'- -CUUGUGCa---AC-UCGUCa----GGGGCGCa-GA- -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 1706 | 0.68 | 0.872677 |
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Target: 5'- uAugGCGUUGGGCAcGUaCUCGCGUUUg -3' miRNA: 3'- cUugUGCAACUCGU-CAgGGGCGCAGA- -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 108987 | 0.68 | 0.857575 |
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Target: 5'- cGAGCGCGUUGAu--GUCCCCGUa--- -3' miRNA: 3'- -CUUGUGCAACUcguCAGGGGCGcaga -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 52993 | 0.68 | 0.857575 |
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Target: 5'- -uGCGCGUacaUGuAGCAGUUcuggCCCGUGUCg -3' miRNA: 3'- cuUGUGCA---AC-UCGUCAG----GGGCGCAGa -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 64850 | 0.68 | 0.849712 |
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Target: 5'- -uACACGUUcgaGAGCGccGUCCCUGCGggCUc -3' miRNA: 3'- cuUGUGCAA---CUCGU--CAGGGGCGCa-GA- -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 39706 | 0.7 | 0.751773 |
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Target: 5'- uGAACGCGUcGAGCAGcgcggccgccaUgUCCGCGUCc -3' miRNA: 3'- -CUUGUGCAaCUCGUC-----------AgGGGCGCAGa -5' |
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| 8035 | 3' | -55 | NC_001973.1 | + | 105143 | 0.71 | 0.74205 |
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Target: 5'- cGACGCGUuaucaccgauUGAcGCAGUCUCCGCG-Cg -3' miRNA: 3'- cUUGUGCA----------ACU-CGUCAGGGGCGCaGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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