Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8039 | 3' | -53.6 | NC_001973.1 | + | 2673 | 0.67 | 0.960199 |
Target: 5'- cGCccGAGCUCGAg----CUCGGCCGAc -3' miRNA: 3'- -CGaaCUUGAGCUaacugGGGCCGGCU- -5' |
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8039 | 3' | -53.6 | NC_001973.1 | + | 58023 | 0.67 | 0.956499 |
Target: 5'- -aUUGAaccGCUCGAUcucgGGCUCCGGCgGc -3' miRNA: 3'- cgAACU---UGAGCUAa---CUGGGGCCGgCu -5' |
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8039 | 3' | -53.6 | NC_001973.1 | + | 133262 | 0.67 | 0.952571 |
Target: 5'- gGCguccAGCUCGGccucGGCCgCGGCCGAg -3' miRNA: 3'- -CGaac-UUGAGCUaa--CUGGgGCCGGCU- -5' |
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8039 | 3' | -53.6 | NC_001973.1 | + | 67784 | 0.67 | 0.947983 |
Target: 5'- gGCUguUGAACUUGAcggagaaUUGGCCggUGGCCGGg -3' miRNA: 3'- -CGA--ACUUGAGCU-------AACUGGg-GCCGGCU- -5' |
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8039 | 3' | -53.6 | NC_001973.1 | + | 75008 | 0.67 | 0.944018 |
Target: 5'- cGCUucaUGuACUCGAgcgUGACCUucacgcggCGGUCGAa -3' miRNA: 3'- -CGA---ACuUGAGCUa--ACUGGG--------GCCGGCU- -5' |
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8039 | 3' | -53.6 | NC_001973.1 | + | 41063 | 0.92 | 0.067554 |
Target: 5'- gGCUUGAACcCGAUcGACCCCGGCCGGc -3' miRNA: 3'- -CGAACUUGaGCUAaCUGGGGCCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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