Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8039 | 3' | -53.6 | NC_001973.1 | + | 40907 | 0.78 | 0.461698 |
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Target: 5'- aUUUGAACUCGA-UGACCCUaGCCGGc -3' miRNA: 3'- cGAACUUGAGCUaACUGGGGcCGGCU- -5' |
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| 8039 | 3' | -53.6 | NC_001973.1 | + | 114953 | 0.78 | 0.443257 |
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Target: 5'- gGCUUGAACUCuGUUaACCCCuGCCGAc -3' miRNA: 3'- -CGAACUUGAGcUAAcUGGGGcCGGCU- -5' |
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| 8039 | 3' | -53.6 | NC_001973.1 | + | 40985 | 0.82 | 0.277877 |
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Target: 5'- aUUUGAACUCGAUUGACCCUuGCCa- -3' miRNA: 3'- cGAACUUGAGCUAACUGGGGcCGGcu -5' |
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| 8039 | 3' | -53.6 | NC_001973.1 | + | 114565 | 0.84 | 0.223632 |
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Target: 5'- gGCUUGAACUCuGUUGACCCUuGCCGGc -3' miRNA: 3'- -CGAACUUGAGcUAACUGGGGcCGGCU- -5' |
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| 8039 | 3' | -53.6 | NC_001973.1 | + | 114722 | 0.9 | 0.098285 |
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Target: 5'- gGUUUGAACUCGAUUGACCCUuGCCGGc -3' miRNA: 3'- -CGAACUUGAGCUAACUGGGGcCGGCU- -5' |
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| 8039 | 3' | -53.6 | NC_001973.1 | + | 41063 | 0.92 | 0.067554 |
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Target: 5'- gGCUUGAACcCGAUcGACCCCGGCCGGc -3' miRNA: 3'- -CGAACUUGaGCUAaCUGGGGCCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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