Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8043 | 5' | -45.8 | NC_001973.1 | + | 95613 | 0.66 | 0.999996 |
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Target: 5'- uGAUGAgcuaCGACUUc--AAGGGGGCGu -3' miRNA: 3'- gCUGCUaa--GCUGAAaacUUUCCUCGC- -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 83827 | 0.66 | 0.999996 |
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Target: 5'- gCGGCGGagcUCGAaucg-GAGAGaGAGCGg -3' miRNA: 3'- -GCUGCUa--AGCUgaaaaCUUUC-CUCGC- -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 65841 | 0.66 | 0.999996 |
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Target: 5'- gGACGAgcccgUCGACga--GGAGGGGGa- -3' miRNA: 3'- gCUGCUa----AGCUGaaaaCUUUCCUCgc -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 22733 | 0.66 | 0.999996 |
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Target: 5'- gGGCGAgccgCGGCcguaucugUgcGAGGGGGGCGa -3' miRNA: 3'- gCUGCUaa--GCUGa-------AaaCUUUCCUCGC- -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 136716 | 0.67 | 0.999977 |
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Target: 5'- gGGCGAg--GAUgcgcGAAAGGAGCGc -3' miRNA: 3'- gCUGCUaagCUGaaaaCUUUCCUCGC- -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 81730 | 0.68 | 0.999899 |
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Target: 5'- aCGACG--UCGGCg-UUGAGcAGGGGCa -3' miRNA: 3'- -GCUGCuaAGCUGaaAACUU-UCCUCGc -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 106680 | 0.69 | 0.999719 |
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Target: 5'- uCGACGAUcUCGACgucgcUUUGgcGGGcGCGc -3' miRNA: 3'- -GCUGCUA-AGCUGa----AAACuuUCCuCGC- -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 133099 | 0.7 | 0.998943 |
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Target: 5'- uGACGcgcUUgGACgug-GAGAGGAGCGc -3' miRNA: 3'- gCUGCu--AAgCUGaaaaCUUUCCUCGC- -5' |
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| 8043 | 5' | -45.8 | NC_001973.1 | + | 85239 | 0.81 | 0.771238 |
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Target: 5'- uGAUGAUguagcggUCGACUUUgGAGAGGGGCGc -3' miRNA: 3'- gCUGCUA-------AGCUGAAAaCUUUCCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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