Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8057 | 3' | -60.9 | NC_001973.1 | + | 128412 | 0.7 | 0.491148 |
Target: 5'- gUCCgUGgcccaCGGCGCCUCC-CUGCCu -3' miRNA: 3'- gAGGaACaa---GCCGCGGAGGcGGCGGc -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 71789 | 0.7 | 0.472953 |
Target: 5'- -gCCgg---CGGCGCCcCCGUCGCCGc -3' miRNA: 3'- gaGGaacaaGCCGCGGaGGCGGCGGC- -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 133895 | 0.72 | 0.371794 |
Target: 5'- -aCCUcGUucUCGGCGCCcucgcgcgUCGCCGCCGc -3' miRNA: 3'- gaGGAaCA--AGCCGCGGa-------GGCGGCGGC- -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 91985 | 0.72 | 0.364052 |
Target: 5'- uCUCCUUG-UCGuguuGCGCUUCCGCCagcuugGCCGc -3' miRNA: 3'- -GAGGAACaAGC----CGCGGAGGCGG------CGGC- -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 25586 | 0.75 | 0.273154 |
Target: 5'- cCUCCUcc-UCGGCGCCcagccugUUCGCCGCCa -3' miRNA: 3'- -GAGGAacaAGCCGCGG-------AGGCGGCGGc -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 109846 | 0.75 | 0.267628 |
Target: 5'- uUCCg----CGGCGUCgCCGCCGCCGa -3' miRNA: 3'- gAGGaacaaGCCGCGGaGGCGGCGGC- -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 71699 | 0.76 | 0.237452 |
Target: 5'- -gCCUUGaaccagcgaacCGGCGCCgCCGCCGCCGc -3' miRNA: 3'- gaGGAACaa---------GCCGCGGaGGCGGCGGC- -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 104251 | 0.8 | 0.127268 |
Target: 5'- gUCCgucagcUGUUCGGCGgCggcgCCGCCGCCGg -3' miRNA: 3'- gAGGa-----ACAAGCCGCgGa---GGCGGCGGC- -5' |
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8057 | 3' | -60.9 | NC_001973.1 | + | 82869 | 0.87 | 0.043383 |
Target: 5'- aCUCCgcgg-CGGCGCCUCCGCCGCCc -3' miRNA: 3'- -GAGGaacaaGCCGCGGAGGCGGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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