Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8058 | 3' | -54.1 | NC_001973.1 | + | 10801 | 0.66 | 0.95224 |
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Target: 5'- --cGUaGGCGC-CGACGgcgGCGAGaAUCg -3' miRNA: 3'- ucaCA-CCGCGaGCUGCa--CGUUC-UAGa -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 26241 | 0.66 | 0.95224 |
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Target: 5'- --gGUGGCGCUCGAcacgacCGUGCGGc---- -3' miRNA: 3'- ucaCACCGCGAGCU------GCACGUUcuaga -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 106966 | 0.66 | 0.948021 |
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Target: 5'- ----gGcGCGCUCGGCGUGCGcGcgCUg -3' miRNA: 3'- ucacaC-CGCGAGCUGCACGUuCuaGA- -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 52164 | 0.67 | 0.938863 |
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Target: 5'- cGGUG-GGCGCcguacuuggCGACGagcgcgGCGGGGUCg -3' miRNA: 3'- -UCACaCCGCGa--------GCUGCa-----CGUUCUAGa -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 87110 | 0.67 | 0.928728 |
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Target: 5'- cGUGUGcGCGUgcgCGGCGUGCGcGcgCc -3' miRNA: 3'- uCACAC-CGCGa--GCUGCACGUuCuaGa -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 106665 | 0.68 | 0.899101 |
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Target: 5'- ----aGGCGCUCGACGcGCucgacGAUCUc -3' miRNA: 3'- ucacaCCGCGAGCUGCaCGuu---CUAGA- -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 160102 | 0.69 | 0.863546 |
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Target: 5'- aAGUGcuuuUGGCGCUUGGCcUGUuuGAUCa -3' miRNA: 3'- -UCAC----ACCGCGAGCUGcACGuuCUAGa -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 41803 | 0.69 | 0.855769 |
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Target: 5'- gAGUG-GGUGCUCGACGUGgAcu-UCg -3' miRNA: 3'- -UCACaCCGCGAGCUGCACgUucuAGa -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 46299 | 0.69 | 0.855769 |
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Target: 5'- cGGUcGUGGCGCUCGGCGc----GAUCg -3' miRNA: 3'- -UCA-CACCGCGAGCUGCacguuCUAGa -5' |
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| 8058 | 3' | -54.1 | NC_001973.1 | + | 134345 | 0.7 | 0.831216 |
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Target: 5'- cGUgGUGGCGCUCGACGc-CGAGGg-- -3' miRNA: 3'- uCA-CACCGCGAGCUGCacGUUCUaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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