Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8063 | 5' | -57.5 | NC_001973.1 | + | 104522 | 0.66 | 0.867404 |
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Target: 5'- -aGCGA-CA-GGUCGCUGAcGAGGUUGa -3' miRNA: 3'- uaUGCUaGUgCCGGCGGCU-CUCCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 4374 | 0.66 | 0.859975 |
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Target: 5'- -gGCGGUCugGcGCU-CCGcGGAGGUCa -3' miRNA: 3'- uaUGCUAGugC-CGGcGGC-UCUCCAGc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 34090 | 0.67 | 0.836517 |
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Target: 5'- -gGCGGUgGCGuaGCCGC--AGAGGUCGu -3' miRNA: 3'- uaUGCUAgUGC--CGGCGgcUCUCCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 16954 | 0.67 | 0.836517 |
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Target: 5'- -cGCGAa-GCGGCCGugcCCGAGuuuuuGGUCGu -3' miRNA: 3'- uaUGCUagUGCCGGC---GGCUCu----CCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 70362 | 0.67 | 0.811444 |
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Target: 5'- -gGCGA--GCGGCCGCU---GGGUCGg -3' miRNA: 3'- uaUGCUagUGCCGGCGGcucUCCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 137875 | 0.68 | 0.79393 |
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Target: 5'- -cGCGGUCAgGGCCGCguCGGcGAGGccCGa -3' miRNA: 3'- uaUGCUAGUgCCGGCG--GCU-CUCCa-GC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 24301 | 0.68 | 0.784957 |
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Target: 5'- -gGCGAcCACGGCgcgcgucgaCGCCGaAGAGGUg- -3' miRNA: 3'- uaUGCUaGUGCCG---------GCGGC-UCUCCAgc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 21490 | 0.76 | 0.361611 |
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Target: 5'- -aGCGcGUCGCGGcCCGCCGAcucGAGGUUGc -3' miRNA: 3'- uaUGC-UAGUGCC-GGCGGCU---CUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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