Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8063 | 5' | -57.5 | NC_001973.1 | + | 105492 | 0.69 | 0.70912 |
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Target: 5'- --cCGggCGCGuuGCCGCCGuaguuGAGGUCGc -3' miRNA: 3'- uauGCuaGUGC--CGGCGGCu----CUCCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 30795 | 0.69 | 0.699264 |
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Target: 5'- -aAUG-UgGCGGUCGcCCGAGGGGUUGg -3' miRNA: 3'- uaUGCuAgUGCCGGC-GGCUCUCCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 32552 | 0.7 | 0.649338 |
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Target: 5'- -gGCGAUCGCcGCCGCCGAcgccGccGUCGg -3' miRNA: 3'- uaUGCUAGUGcCGGCGGCU----CucCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 104269 | 0.7 | 0.649338 |
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Target: 5'- -gGCGG-CGCcGCCGCCGGGcuGGUCGu -3' miRNA: 3'- uaUGCUaGUGcCGGCGGCUCu-CCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 83934 | 0.7 | 0.639281 |
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Target: 5'- -aGCGGaCACGGCgCGCCGcucGGAGG-CGg -3' miRNA: 3'- uaUGCUaGUGCCG-GCGGC---UCUCCaGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 112626 | 0.71 | 0.618152 |
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Target: 5'- uGUAUGGUCACGGCCcaGCCGuAGGcgagcggcaagccGGUCa -3' miRNA: 3'- -UAUGCUAGUGCCGG--CGGC-UCU-------------CCAGc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 38136 | 0.72 | 0.520177 |
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Target: 5'- -cACGAaCGCGGCuuggauggggccCGCCGAGAGGaCGg -3' miRNA: 3'- uaUGCUaGUGCCG------------GCGGCUCUCCaGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 21490 | 0.76 | 0.361611 |
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Target: 5'- -aGCGcGUCGCGGcCCGCCGAcucGAGGUUGc -3' miRNA: 3'- uaUGC-UAGUGCC-GGCGGCU---CUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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