Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8064 | 3' | -58.4 | NC_001973.1 | + | 17592 | 0.66 | 0.806399 |
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Target: 5'- --gUCGGCGUGCUGGCauauuuccgUGcacaaaUUGAGCGu -3' miRNA: 3'- auaGGCCGCACGGCCG---------AC------AACUCGCu -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 124200 | 0.66 | 0.797572 |
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Target: 5'- gGUCgCGGCGgcgcGCCGGCUcgUGAcCGAc -3' miRNA: 3'- aUAG-GCCGCa---CGGCCGAcaACUcGCU- -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 82508 | 0.66 | 0.788599 |
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Target: 5'- --gCCGGCGUcgcgGCCGGCcugcaGUUGGGUu- -3' miRNA: 3'- auaGGCCGCA----CGGCCGa----CAACUCGcu -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 39882 | 0.66 | 0.779489 |
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Target: 5'- --cUCGGCGgcaccgGCCGGUUGUUGu-CGAa -3' miRNA: 3'- auaGGCCGCa-----CGGCCGACAACucGCU- -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 146113 | 0.67 | 0.770248 |
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Target: 5'- ---gCGGCGcugaGCCGGCUcggGAGCGGg -3' miRNA: 3'- auagGCCGCa---CGGCCGAcaaCUCGCU- -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 94533 | 0.67 | 0.751416 |
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Target: 5'- --cCCGGCGcGCCGGUcaucgacUGGGCGGa -3' miRNA: 3'- auaGGCCGCaCGGCCGaca----ACUCGCU- -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 10703 | 0.67 | 0.732172 |
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Target: 5'- gAUCuCGGCG-GgCGGCUcGaUGAGCGGc -3' miRNA: 3'- aUAG-GCCGCaCgGCCGA-CaACUCGCU- -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 81811 | 0.7 | 0.56179 |
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Target: 5'- gUGUCCGGCGccGCCGGCggcgacGAGaCGAc -3' miRNA: 3'- -AUAGGCCGCa-CGGCCGacaa--CUC-GCU- -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 95720 | 0.71 | 0.522424 |
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Target: 5'- ---gCGGUGcGCCGGCUGUUG-GCGc -3' miRNA: 3'- auagGCCGCaCGGCCGACAACuCGCu -5' |
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| 8064 | 3' | -58.4 | NC_001973.1 | + | 158676 | 0.73 | 0.40352 |
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Target: 5'- aGUCCGGCGaaGCCGGCgGccGAGCGc -3' miRNA: 3'- aUAGGCCGCa-CGGCCGaCaaCUCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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