Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8065 | 5' | -55.2 | NC_001973.1 | + | 39388 | 0.67 | 0.931634 |
Target: 5'- aGGucacCUGcUCGACGGCgCGCGCcaGGu -3' miRNA: 3'- -CCuc--GACaAGCUGCCGaGCGCGaaCC- -5' |
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8065 | 5' | -55.2 | NC_001973.1 | + | 146009 | 0.67 | 0.915421 |
Target: 5'- gGGAGCggGUcCGcCGGCUCGaGCgcgGGu -3' miRNA: 3'- -CCUCGa-CAaGCuGCCGAGCgCGaa-CC- -5' |
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8065 | 5' | -55.2 | NC_001973.1 | + | 60405 | 0.67 | 0.90955 |
Target: 5'- aGGAGCUGaUCuugcaGCGGg-CGCGCUcgcUGGa -3' miRNA: 3'- -CCUCGACaAGc----UGCCgaGCGCGA---ACC- -5' |
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8065 | 5' | -55.2 | NC_001973.1 | + | 95360 | 0.73 | 0.620221 |
Target: 5'- uGGaAGCUGUUCGACGGcCUgGaCGUggacUGGg -3' miRNA: 3'- -CC-UCGACAAGCUGCC-GAgC-GCGa---ACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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