Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8065 | 5' | -55.2 | NC_001973.1 | + | 46073 | 0.7 | 0.786085 |
Target: 5'- -uGGCUGUUCGGacugacgUGGCUCGUGCg--- -3' miRNA: 3'- ccUCGACAAGCU-------GCCGAGCGCGaacc -5' |
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8065 | 5' | -55.2 | NC_001973.1 | + | 3567 | 0.72 | 0.690894 |
Target: 5'- cGGGGCgcgcgaUGacCGACGGCgCGCGCUUcGGc -3' miRNA: 3'- -CCUCG------ACaaGCUGCCGaGCGCGAA-CC- -5' |
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8065 | 5' | -55.2 | NC_001973.1 | + | 104257 | 0.73 | 0.640486 |
Target: 5'- -cAGCUGUUCGGCGGCg-GCGCc--- -3' miRNA: 3'- ccUCGACAAGCUGCCGagCGCGaacc -5' |
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8065 | 5' | -55.2 | NC_001973.1 | + | 95360 | 0.73 | 0.620221 |
Target: 5'- uGGaAGCUGUUCGACGGcCUgGaCGUggacUGGg -3' miRNA: 3'- -CC-UCGACAAGCUGCC-GAgC-GCGa---ACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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