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miRNA: 3'- -GUCCCGGGUCUGAUUCCGAGACUGCA- -5'
miRNA: 3'- -GUCCCGGGUCUGAUUCCGAGACUGCA- -5'
miRNA: 3'- gucCCGGGUCUGAuUCCGaGACUGCA- -5'
miRNA: 3'- gucCCGGGUCUGAuUCCGaGACUGCA- -5'
miRNA: 3'- gucCCGGGUCugAUUCCGAG----ACUGca -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 807 | 3' | -57.1 | NC_000858.1 | + | 88 | 1.06 | 6.7e-05 |
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Target: 5'- uCAGGGCCCAGACUAAGGCUCUGACGUc -3' miRNA: 3'- -GUCCCGGGUCUGAUUCCGAGACUGCA- -5' |
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| 807 | 3' | -57.1 | NC_000858.1 | + | 8353 | 1.06 | 6.7e-05 |
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Target: 5'- uCAGGGCCCAGACUAAGGCUCUGACGUc -3' miRNA: 3'- -GUCCCGGGUCUGAUUCCGAGACUGCA- -5' |
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| 807 | 3' | -57.1 | NC_000858.1 | + | 140 | 0.74 | 0.036986 |
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Target: 5'- --cGGCUCAGACU-AGGCcCUGACGUa -3' miRNA: 3'- gucCCGGGUCUGAuUCCGaGACUGCA- -5' |
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| 807 | 3' | -57.1 | NC_000858.1 | + | 8405 | 0.74 | 0.036986 |
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Target: 5'- --cGGCUCAGACU-AGGCcCUGACGUa -3' miRNA: 3'- gucCCGGGUCUGAuUCCGaGACUGCA- -5' |
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| 807 | 3' | -57.1 | NC_000858.1 | + | 2677 | 0.66 | 0.156447 |
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Target: 5'- --uGGCCCGGugUAGGGUUCgauaUGGCc- -3' miRNA: 3'- gucCCGGGUCugAUUCCGAG----ACUGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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