miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8072 5' -55.9 NC_001978.2 + 27240 0.68 0.45168
Target:  5'- uUCUUCCCGg----GCGUGCUcgACGCu -3'
miRNA:   3'- gAGAAGGGCaagaaCGCGCGG--UGCGu -5'
8072 5' -55.9 NC_001978.2 + 39867 0.66 0.60206
Target:  5'- gCUCUUCaagggCGUUCagggcgucgGCGCGCUcCGCAa -3'
miRNA:   3'- -GAGAAGg----GCAAGaa-------CGCGCGGuGCGU- -5'
8072 5' -55.9 NC_001978.2 + 5777 0.67 0.514089
Target:  5'- -aCUUCgCGUUCUUGCGguaCGCCgACGgGa -3'
miRNA:   3'- gaGAAGgGCAAGAACGC---GCGG-UGCgU- -5'
8072 5' -55.9 NC_001978.2 + 5052 0.71 0.33236
Target:  5'- gCUCUUCCU--UCUcGCGCGCCucaGCGg -3'
miRNA:   3'- -GAGAAGGGcaAGAaCGCGCGGug-CGU- -5'
8072 5' -55.9 NC_001978.2 + 7061 0.72 0.288586
Target:  5'- gUCUUCCCGcccgaugccaaugCUUGCGUcgaguGCCAUGCAa -3'
miRNA:   3'- gAGAAGGGCaa-----------GAACGCG-----CGGUGCGU- -5'
8072 5' -55.9 NC_001978.2 + 1587 0.97 0.004527
Target:  5'- gCUCUUCCCGUUCUUG-GCGCCACGCAc -3'
miRNA:   3'- -GAGAAGGGCAAGAACgCGCGGUGCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.