Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8079 | 3' | -53.6 | NC_001978.2 | + | 31374 | 0.72 | 0.427468 |
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Target: 5'- aGCGAAGGaaUGCugaacgguGCUGAucACGUCGCCguCGGc -3' miRNA: 3'- -CGCUUCC--ACG--------UGACU--UGCAGUGG--GCC- -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 25782 | 0.72 | 0.427468 |
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Target: 5'- cCGAuacgGCAgUGAgcGCGUCGCCCGGa -3' miRNA: 3'- cGCUuccaCGUgACU--UGCAGUGGGCC- -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 6530 | 0.72 | 0.408275 |
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Target: 5'- cGCGAAGGUGCgACUGuuCGaaGCCCu- -3' miRNA: 3'- -CGCUUCCACG-UGACuuGCagUGGGcc -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 10566 | 0.72 | 0.389629 |
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Target: 5'- cGCGAAGGacGCACUGAACGcggucgucaauuUCGugaaggggcuuCCCGGu -3' miRNA: 3'- -CGCUUCCa-CGUGACUUGC------------AGU-----------GGGCC- -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 1659 | 0.74 | 0.305115 |
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Target: 5'- aGCGAugaucUGuCGCUGAGCGUCGCCcCGGu -3' miRNA: 3'- -CGCUucc--AC-GUGACUUGCAGUGG-GCC- -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 27277 | 0.75 | 0.282661 |
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Target: 5'- gGCGAGc--GCAUcGGACGUCGCCCGGu -3' miRNA: 3'- -CGCUUccaCGUGaCUUGCAGUGGGCC- -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 7761 | 0.75 | 0.275473 |
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Target: 5'- aGCGAAGcG-GCGCUGAAC-UCACCCu- -3' miRNA: 3'- -CGCUUC-CaCGUGACUUGcAGUGGGcc -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 8827 | 0.76 | 0.241715 |
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Target: 5'- gGCGAAGcGgggGCACUGAGCGccgcaCGCCCGu -3' miRNA: 3'- -CGCUUC-Ca--CGUGACUUGCa----GUGGGCc -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 33495 | 0.76 | 0.229208 |
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Target: 5'- cGCGAAGGUGCGgUauGCGg-ACCCGGa -3' miRNA: 3'- -CGCUUCCACGUgAcuUGCagUGGGCC- -5' |
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| 8079 | 3' | -53.6 | NC_001978.2 | + | 6636 | 1.12 | 0.000726 |
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Target: 5'- cGCGAAGGUGCACUGAACGUCACCCGGc -3' miRNA: 3'- -CGCUUCCACGUGACUUGCAGUGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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