Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8080 | 5' | -56.8 | NC_001978.2 | + | 41146 | 0.66 | 0.485259 |
Target: 5'- -gUgUGAAgaGCCAUGCgaaGCGGCGUGCUg -3' miRNA: 3'- uaGgGCUUa-CGGUAUG---UGCCGCGCGA- -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 38643 | 0.72 | 0.220607 |
Target: 5'- cGUCCCGAAcGCCgacGUGCcCGGCGCuucGCUg -3' miRNA: 3'- -UAGGGCUUaCGG---UAUGuGCCGCG---CGA- -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 37292 | 0.67 | 0.432393 |
Target: 5'- -gCCCG-AUGCCuaggucuucgcACAUGGCGCGUUc -3' miRNA: 3'- uaGGGCuUACGGua---------UGUGCCGCGCGA- -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 35249 | 0.71 | 0.258823 |
Target: 5'- gAUCCUGAAcccGUCGUAC-CGGgGCGCUg -3' miRNA: 3'- -UAGGGCUUa--CGGUAUGuGCCgCGCGA- -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 34272 | 0.66 | 0.538826 |
Target: 5'- uUgCCGGAgccacGCUGUACACGaCGCGCg -3' miRNA: 3'- uAgGGCUUa----CGGUAUGUGCcGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 33860 | 0.71 | 0.265695 |
Target: 5'- -aCCCGggUGCCG-AC-CGuGCGCGUg -3' miRNA: 3'- uaGGGCuuACGGUaUGuGC-CGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 27650 | 0.66 | 0.482123 |
Target: 5'- cAUCCCGGAgacugagcGCgaccuucgugaguuCAUGCACugGGCGCGCa -3' miRNA: 3'- -UAGGGCUUa-------CG--------------GUAUGUG--CCGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 24150 | 0.66 | 0.517124 |
Target: 5'- cUUCCGAAUGaucCCGUcggacGCACGG-GCGCg -3' miRNA: 3'- uAGGGCUUAC---GGUA-----UGUGCCgCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 22944 | 0.7 | 0.287196 |
Target: 5'- -aCCUGAgccccucaGUGCCuUACACGG-GCGCUg -3' miRNA: 3'- uaGGGCU--------UACGGuAUGUGCCgCGCGA- -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 19362 | 0.71 | 0.245515 |
Target: 5'- cGUCCCGAcggacgccgacGUGaCGUGCaaGCGGUGCGCg -3' miRNA: 3'- -UAGGGCU-----------UACgGUAUG--UGCCGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 19305 | 0.66 | 0.538826 |
Target: 5'- -gCCCGAcgGCUucaugGCuucccCGGCGUGCg -3' miRNA: 3'- uaGGGCUuaCGGua---UGu----GCCGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 12343 | 0.72 | 0.239078 |
Target: 5'- gGUCCCGAcacgGCCGgacaACGCGGCG-GCa -3' miRNA: 3'- -UAGGGCUua--CGGUa---UGUGCCGCgCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 10931 | 0.66 | 0.485259 |
Target: 5'- -aCCCGGAcGCCGguccaccCAUGGCGCGa- -3' miRNA: 3'- uaGGGCUUaCGGUau-----GUGCCGCGCga -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 6826 | 1.05 | 0.000811 |
Target: 5'- aAUCCCGAAUGCCAUACACGGCGCGCUc -3' miRNA: 3'- -UAGGGCUUACGGUAUGUGCCGCGCGA- -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 6783 | 0.66 | 0.517124 |
Target: 5'- -gCCCGGc-GUCGUGacaACGGCGUGCg -3' miRNA: 3'- uaGGGCUuaCGGUAUg--UGCCGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 6662 | 0.67 | 0.474845 |
Target: 5'- -cCCCGug-GCUAUGCGCuccGGCGCGa- -3' miRNA: 3'- uaGGGCuuaCGGUAUGUG---CCGCGCga -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 2587 | 0.67 | 0.434364 |
Target: 5'- -gCCUGAGUGgCAgGCACcuGCGCGCg -3' miRNA: 3'- uaGGGCUUACgGUaUGUGc-CGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 2383 | 0.66 | 0.495781 |
Target: 5'- cGUCCgCGAAgaGCUAcACACGGCGCu-- -3' miRNA: 3'- -UAGG-GCUUa-CGGUaUGUGCCGCGcga -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 879 | 0.67 | 0.45436 |
Target: 5'- uGUCCgaauggguCGAGUGCuCcgGCGCcugGGCGCGCg -3' miRNA: 3'- -UAGG--------GCUUACG-GuaUGUG---CCGCGCGa -5' |
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8080 | 5' | -56.8 | NC_001978.2 | + | 647 | 0.66 | 0.538826 |
Target: 5'- uUgCUGAAcGCggCGUACACGGCGCGg- -3' miRNA: 3'- uAgGGCUUaCG--GUAUGUGCCGCGCga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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