miRNA: 3'- -GUGCCCuuuCGAGuuGCCGGCAUgCG- -5'
miRNA: 3'- -GUGCccUuuCGAGUUGc---CGGCAUGCG- -5'
miRNA: 3'- gUGCCCuuuCGAGUUGCcgGCAUGCG- -5'
miRNA: 3'- -GUGCC-CU-----UUCGAGUUGC-CGGCAUGcg -5'
miRNA: 3'- gUGCCC------UU--------------UCGAGUU-GCCGGCa-UGCG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8083 | 5' | -54 | NC_001978.2 | + | 33092 | 0.67 | 0.671079 |
|
Target: 5'- uCGCGGGcuuGCUUc-CGGCCGUA-GCc -3' miRNA: 3'- -GUGCCCuuuCGAGuuGCCGGCAUgCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 32283 | 0.66 | 0.693405 |
|
Target: 5'- gCGCGcuGccGCUCAGCcucaGCCGUGCGCc -3' miRNA: 3'- -GUGCccUuuCGAGUUGc---CGGCAUGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 25982 | 0.66 | 0.693405 |
|
Target: 5'- aACGGGcacGUUCAGCG--CGUACGCg -3' miRNA: 3'- gUGCCCuuuCGAGUUGCcgGCAUGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 23595 | 0.66 | 0.70448 |
|
Target: 5'- uCACGGuGAuugccGGGUUCGGCGcGUCGUACu- -3' miRNA: 3'- -GUGCC-CU-----UUCGAGUUGC-CGGCAUGcg -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 39447 | 0.66 | 0.733979 |
|
Target: 5'- aGCGGGcucgauGAucgguccgcaaucaAGCUCGAcCGGCCGgaGCGUg -3' miRNA: 3'- gUGCCC------UU--------------UCGAGUU-GCCGGCa-UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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